Gespeichert in:
| Titel: | Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins |
|---|---|
| Weiterer Titel: | Aufklärung der molekularen Mechanismen der Interaktion zwischen dem cytomegaloviralen CDK-Ortholog pUL97 und zellulären Cyclinen |
| Von: |
vorgelegt von Mirjam Claudia Steingruber
|
| Person: |
Steingruber, Mirjam Claudia
Verfasser aut |
| Hauptverfasser: | |
| Format: | Abschlussarbeit Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
Erlangen ; Nürnberg
2019
|
| Schlagworte: | |
| Online-Zugang: | http://d-nb.info/1191784924/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031521293&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | 87 Seiten Illustrationen 21 cm |
Internformat
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| 100 | 1 | |a Steingruber, Mirjam Claudia |e Verfasser |0 (DE-588)119781261X |4 aut | |
| 245 | 1 | 0 | |a Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins |c vorgelegt von Mirjam Claudia Steingruber |
| 246 | 1 | 3 | |a Aufklärung der molekularen Mechanismen der Interaktion zwischen dem cytomegaloviralen CDK-Ortholog pUL97 und zellulären Cyclinen |
| 264 | 1 | |a Erlangen ; Nürnberg |c 2019 | |
| 300 | |a 87 Seiten |b Illustrationen |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Alexander-Universität Erlangen-Nürnberg |d 2019 | ||
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-031521293 | |
Datensatz im Suchindex
| DE-BY-UBR_call_number | 23/19.1527 |
|---|---|
| DE-BY-UBR_katkey | 6173749 |
| DE-BY-UBR_location | UB Magazin |
| DE-BY-UBR_media_number | 069040889677 |
| _version_ | 1835101714159501313 |
| adam_text | TABLE
OF
CONTENTS
1.
SUMMARY
................................................................................................................................
1
2.
ZUSAMMENFASSUNG
.............................................................................................................
2
3.
INTRODUCTION
.......................................................................................................................
3
3.1.
THE
HUMAN
CYTOMEGALOVIRUS
...........................................................................................
3
3.2
CELL
CYCLE
MODULATION
BY
HCMV........................................................................................
5
3.3
CDKS
AND
CYCLING
................................................................................................................
7
3.4
CYCLINS
BL,
H
AND
T1
........................................................................................................
10
3.5
VIRAL
PROTEIN
KINASE
PUL97
-
A
CDK
ORTHOLOG
..................................................................
12
4.
OBJECTIVES
...........................................................................................................................
15
5.
MATERIAL
&
METHODS
........................................................................................................
16
5.1
BIOLOGICAL
MATERIAL
..........................................................................................................
16
5.2
MEDIA,
BUFFER
AND
REAGENTS
..............................................................
18
5.3
NUCLEIC
ACIDS
....................................................................................................................
21
5.4
SITE-DIRECTED
MUTAGENESIS
.............................................................................................
22
5.5
CELL
CULTURE
TECHNIQUES
...................................................................................................
22
5.6
PEI
TRANSFECTION
...............................................................................................................
23
5.7
VIRUS
INFECTION
..........................................................................................
23
5.8
COIMMUNOPRECIPITATION
AND
IN
VITRO
KINASE
ASSAYS
.......................................................
23
5.9
PROTEIN
DEPLETION-
AND
ASSEMBLY-BASED
COIP................................................................
24
5.10
ELUTION
OF
MYC-CYCLIN
T1
.........
......................................................................................
24
5.11
PHOSPHATASE
ASSAY
........................................................................................................
25
5.12
PROTEOMIC
ANALYSES
.......................................................................................................
26
5.13
INDIRECT
IMMUNOFLUORESCENCE
ANALYSES
.......................................................................
26
5.14
BIOINFORMATIC
ANALYSES.
.........................................................................................
27
6.
RESULTS
..................................................................................................................................
28
6.1
HCMV
PROTEIN
KINASE
PUL97
INTERACTS
WITH
THREE
DIFFERENT
TYPES
OF
CYCLINS
..............
28
6.1.1
THE
MODE
OF
PUL97
BINDING
TO
CYCLINS
BL,
T1
AND
H
IS
CYCLIN
TYPE-SPECIFIC
...........
28
6.1.2
CYCLINS
BL,
T1
AND
H,
BUT
NOT
CYCLINS
B2
OR
DI,
COLOCALIZE
WITH
PUL97
IN
EARLY
VIRAL
REPLICATION
COMPARTMENTS
...............................................................................................
31
6.1.3
PUL97-CYCLIN
INTERACTION
OCCURS
INDEPENDENT
OF
CELL
TYPE
AND
HCMV
STRAIN
........
34
6.1.4
PROTEIN
COMPLEXES
BASED
ON
PUL97-CYDIN
INTERACTION
CONTAIN
SUBSTRATES
OF
PUL97
....................................................................................................................................
35
6.1.5
CYCLIN
BL
INTERACTION
IS
CONSERVED
AMONG
P-,
BUT
NOT
A-
OR
Y-HERPESVIRAL
KINASES
38
6.2
THE
PUL97
INTERACTION
WITH
CYCLIN
BL
IS
DETERMINED
BY
UNIQUE
BINDING
FEATURES
.....
39
6.2.1
PUL97-CYCLIN
BL
INTERACTION
OCCURS
IN
A
KINASE
ACTIVITY-DEPENDENT
MANNER
.........
39
6.2.2
PUL97-CYCLIN
BL
INTERACTION
IS
DETERMINED
BY
THE
STATE
OF
PHOSPHORYLATION
........
41
6.2.3
CYCLIN
BL,
BUT
NOT
CYCLIN
H,
IS
PHOSPHORYLATED
BY
PUL97
IN
VITRO
..............................
43
6.2.4
MUTAGENESIS
OF
PUL97
DEMONSTRATES
A
CORRELATION
BETWEEN
PUL97
ACTIVITY
AND
CYCLIN
BL
BINDING
.................................................................................................................
44
6.2.5
DETERMINATION
OF
PHOSPHOSITES
IN
PUL97
APPLYING
MS-BASED
PHOSPHOSITE
ANALYSIS
48
6.3
FUNCTIONAL
CONSEQUENCES
OF
PUL97-CYCLIN
INTERACTION
..................................................
50
6.3.1
CYCLIN
T1
AND
H
ARE
SUBSTANTIALLY
INVOLVED
IN
PUL97-PUL97
SELF-INTERACTION
........
50
6.3.2
CYCLIN-PUL97
COMPLEXES
COMPRISE
BINDING
TO
VARIOUS
SUBSTRATES
BUT
DO
NOT
DETERMINE
STRICT
SUBSTRATE
SPECIFICITY
.............................................................................
56
6.3.3
BINARY
AND
TERNARY
COMPLEXES
COMPRISING
PUL97-CYDIN-H-CDK7
..........................
59
7.
DISCUSSION
............................................................................................................................
64
7.1
CHARACTERIZATION
OF
PUL97-CYCLIN
INTERACTION
............... ................................................
64
7.2
FUNCTIONAL
RELEVANCE
OF
PUL97-CYCLIN
INTERACTIONS
........................................................
67
7.3
COMMON
AND
DIFFERENT
PROPERTIES
OF
VCDK
PUL97
AND
HUMAN
CDKS
..........................
68
8.
REFERENCES
...........................................................................................................................
73
9.
APPENDIX
..............................................................................................................................
82
9.1
LIST
OF
ABBREVIATION
.........................................................................................................
82
9.2
MASS
SPECTROMETRY
DATA
.................................................................................................
84
9.3
OWN
PUBLICATIONS
.............................................................................................................
86
9.4
ACKNOWLEDGMENTS
.........................................................................................................
87
|
| any_adam_object | 1 |
| author | Steingruber, Mirjam Claudia |
| author_GND | (DE-588)119781261X |
| author_facet | Steingruber, Mirjam Claudia |
| author_role | aut |
| author_sort | Steingruber, Mirjam Claudia |
| author_variant | m c s mc mcs |
| building | Verbundindex |
| bvnumber | BV046141052 |
| ctrlnum | (OCoLC)1119094177 (DE-599)DNB1191784924 |
| discipline | Medizin |
| format | Thesis Book |
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| id | DE-604.BV046141052 |
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| indexdate | 2024-12-20T18:44:10Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-031521293 |
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| physical | 87 Seiten Illustrationen 21 cm |
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| spellingShingle | Steingruber, Mirjam Claudia Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins Cycline (DE-588)4313464-6 gnd Proteinkinasen (DE-588)4175996-5 gnd Cyclin-abhängige Kinasen (DE-588)4485596-5 gnd Cytomegalie-Virus (DE-588)4238363-8 gnd Protein-Protein-Wechselwirkung (DE-588)4745193-2 gnd |
| subject_GND | (DE-588)4313464-6 (DE-588)4175996-5 (DE-588)4485596-5 (DE-588)4238363-8 (DE-588)4745193-2 (DE-588)4113937-9 |
| title | Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins |
| title_alt | Aufklärung der molekularen Mechanismen der Interaktion zwischen dem cytomegaloviralen CDK-Ortholog pUL97 und zellulären Cyclinen |
| title_auth | Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins |
| title_exact_search | Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins |
| title_full | Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins vorgelegt von Mirjam Claudia Steingruber |
| title_fullStr | Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins vorgelegt von Mirjam Claudia Steingruber |
| title_full_unstemmed | Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins vorgelegt von Mirjam Claudia Steingruber |
| title_short | Molecular mode of interaction between the cytomegaloviral CDK ortholog pUL97 and cellular cyclins |
| title_sort | molecular mode of interaction between the cytomegaloviral cdk ortholog pul97 and cellular cyclins |
| topic | Cycline (DE-588)4313464-6 gnd Proteinkinasen (DE-588)4175996-5 gnd Cyclin-abhängige Kinasen (DE-588)4485596-5 gnd Cytomegalie-Virus (DE-588)4238363-8 gnd Protein-Protein-Wechselwirkung (DE-588)4745193-2 gnd |
| topic_facet | Cycline Proteinkinasen Cyclin-abhängige Kinasen Cytomegalie-Virus Protein-Protein-Wechselwirkung Hochschulschrift |
| url | http://d-nb.info/1191784924/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=031521293&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT steingrubermirjamclaudia molecularmodeofinteractionbetweenthecytomegaloviralcdkorthologpul97andcellularcyclins AT steingrubermirjamclaudia aufklarungdermolekularenmechanismenderinteraktionzwischendemcytomegaloviralencdkorthologpul97undzellularencyclinen |
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