Verfügbarkeit: "Analyses of the urinary miRNA-expression of triple-negative breast cancer patients"

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Titel:	"Analyses of the urinary miRNA-expression of triple-negative breast cancer patients"
Von:	vorgelegt von Dr.-Ing. Sebastian Günther
Person:	Günther, Sebastian Verfasser aut
Hauptverfasser:	Günther, Sebastian (VerfasserIn)
Format:	Abschlussarbeit Buch
Sprache:	Englisch
Veröffentlicht:	Aachen [2021]
Schlagworte:	Hochschulschrift
Online-Zugang:	https://d-nb.info/1232033286/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033269126&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
Beschreibung:	X, 84 Seiten, XLI Illustrationen, Diagramme 21 cm

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Datensatz im Suchindex

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adam_text	CONTENT 1. INTRODUCTION ..................................................................................................................... 1 1.1 BREAST CANCER ............................................................................................................. 1 1.1.1 EPIDEMIOLOGY OF BREAST CANCER .......................................................................... 1 1.1.2 CLASSIFICATION OF BREAST CANCER ........................................................................... 1 1.1.3 BREAST CANCER STEM CELLS .................................................................................... 2 1.2 MICRO RNA INVOLVEMENT IN BREAST CANCER DEVELOPMENT AND PROGRESSION ............ 4 1.2.1 MICRO RNA BIOGENESIS AND FUNCTIONS ................................................................ 4 1.2.2 MICRO RNAS IN CANCER DEVELOPMENT AND PROGRESSION IN GENERAL ................... 5 1.2.3 MICRO RNAS IN BREAST CANCER DEVELOPMENT AND PROGRESSION .......................... 6 1.2.4 URINARY MICRO RNAS IN BREAST CANCER DEVELOPMENT AND PROGRESSION ............ 8 2. OBJECTIVE OF THIS THESIS .................................................................................................. 10 3. MATERIAL AND METHODS ............................................................................................. 12 3.1 URINE SAMPLES .......................................................................................................... 12 3.2 TOTAL-RNA ISOLATION FROM URINE SAMPLES ................................................................. 12 3.3 GENECHIPYY MIRNA 4.0 BASED MIRNA EXPRESSION MEASUREMENT .......................... 12 3.4 DATA AND MIRNA ANALYSES ....................................................................................... 13 4. RESULTS ............................................................................................................................ 14 4.1 URINARY MIRNAS CAN DISTINGUISH TNBC PATIENTS FROM BREAST HEALTHY WOMEN .... 14 4.1.1 MICROARRAY DATA ANALYSIS .................................................................................. 14 4.1.2 LITERATURE-RESEARCH ON INVOLVEMENT OF DYSREGULATED MIRNAS IN BC ............ 17 4.1.3. INFLUENCE OF UPREGULATED MIRNAS ON BC DEVELOPMENT AND PROGRESSION . 18 4.1.4 INFLUENCE OF DOWNREGULATED MIRNAS ON BC DEVELOPMENT AND PROGRESSION. 20 4.1.5 INTERIM CONCLUSION .......................................................................................... 56 4.2 NEOADJUVANT CHEMOTHERAPY CHANGES URINARY MIRNA SIGNATURES AND REFLECTS CHEMOTHERAPEUTIC STATUS IN TNBC PATIENTS ................................................................ 60 4.2.1 MICROARRAY DATA ANALYSIS .................................................................................. 60 4.2.2 INFLUENCE OF MIRNAS HIGHER EXPRESSED IN NATIVE TNBC URINES AS COMPARED TO POST-CHEMOTHERAPY URINES ON BC DEVELOPMENT AND PROGRESSION .......................... 61 4.2.3 INFLUENCE OF MIRNAS LOWER EXPRESSED IN NATIVE TNBC URINES AS COMPARED TO POST-CHEMOTHERAPY TNBC URINES ON BC DEVELOPMENT AND PROGRESSION ............... 61 4.2.4 INTERIM CONCLUSION ............................................................................................ 63 4.3 NEOADJUVANT CHEMOTHERAPY ALIGNS MIRNA SIGNATURES OF TNBC PATIENTS AND HEALTHY DONORS ............................................................................................................... 64 4.3.1 MICROARRAY DATA ANALYSIS .................................................................................. 64 4.3.2 ASSESSMENT OF MIRNAS UPREGULATED IN POST-CHEMOTHERAPY TNBC URINES ....64 4.3.3 ASSESSMENT OF MIRNAS DOWNREGULATED IN POST-CHEMOTHERAPY TNBC URINES65 4.3.4. INTERIM CONCLUSION ...................................................................................... 66 4.4 INTERMEDIATE CONCLUSION ON RESULTS OBTAINED ....................................................... 66 4.5 PATHWAY ANALYSIS FOR DYSREGULATED MIRNAS BETWEEN TNBC PATIENTS AND HEALTHY WOMEN ........................................................................................................................... 69 4.5.1 GENES UNION ..................................................................................................... 69 4.5.2 GENES INTERSECTION ........................................................................................... 71 4.5.3 PATHWAYS UNION ................................................................................................ 71 4.5.4 PATHWAYS INTERSECTION ...................................................................................... 72 4.5.5 INTERIM CONCLUSION ............................................................................................ 73 5. DISCUSSION .................................................................................................................. 74 5.1 GENERAL ASPECTS OF URINARY MIRNA EXPRESSION IN TRIPLE-NEGATIVE BREAST CANCER ..74 5.2 URINARY MIRNA-BASED DETECTION OF TNBC PATIENTS USING DYSREGULATED MIRNAS. .74 5.2.1 MIRNAS UPREGULATED IN NATIVE TNBC URINES .................................................... 74 5.2.2 MIRNAS DOWNREGULATED IN NATIVE TNBC URINES ............................................... 76 5.2.3 INTERIM CONCLUSION ............................................................................................ 77 5.3 URINARY MIRNA-BASED CONTROL OF TNBC PATIENTS RESPONSE TO NEOADJUVANT CHEMOTHERAPY ............................................................................................................... 77 5.3.1 MIRNAS HIGHER EXPRESSED IN NATIVE TNBC AS COMPARED TO POST CHEMOTHERAPY URINES ................................................................................................ 77 5.3.2 MIRNAS LOWER EXPRESSED IN NATIVE TNBC AS COMPARED TO POST-CHEMOTHERAPY TNBC URINES ................................................................................................................ 78 5.3.3 INTERIM CONCLUSION ........................................................................................... 78 5.4 MIRNA SIGNATURES OF POST-CHEMOTHERAPY AND HEALTHY DONOR URINES ARE ALMOST EQUAL .............................................................................................................................. 80 5.4.1 MIRNAS UPREGULATED IN POST-CHEMOTHERAPY TNBC URINES ............................. 80 5.4.2 MIRNAS DOWNREGULATED IN POST-CHEMOTHERAPY TNBC URINES ....................... 81 5.4.3. INTERIM CONCLUSION .......................................................................................... 81 6. CONCLUSION ...................................................................................................................... 82 7. FUTURE PROSPECTS ........................................................................................................... 84 7.1 EXPERIMENTAL SETUP ................................................................................................. 84 7.2 RAISE OF SAMPLE NUMBERS ........................................................................................ 84 7.3 DATA VALIDATION BY RT QPCR BASED ON URINARY TOTAL-RNA/CDNA ............................ 84 7.4 VALIDATION COHORT .................................................................................................... 84 7.5 RESEARCH OF MIRNA EXPRESSION IN TNBC STEM CELLS ............................................... 84
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spellingShingle	Günther, Sebastian "Analyses of the urinary miRNA-expression of triple-negative breast cancer patients"
subject_GND	(DE-588)4113937-9
title	"Analyses of the urinary miRNA-expression of triple-negative breast cancer patients"
title_auth	"Analyses of the urinary miRNA-expression of triple-negative breast cancer patients"
title_exact_search	"Analyses of the urinary miRNA-expression of triple-negative breast cancer patients"
title_full	"Analyses of the urinary miRNA-expression of triple-negative breast cancer patients" vorgelegt von Dr.-Ing. Sebastian Günther
title_fullStr	"Analyses of the urinary miRNA-expression of triple-negative breast cancer patients" vorgelegt von Dr.-Ing. Sebastian Günther
title_full_unstemmed	"Analyses of the urinary miRNA-expression of triple-negative breast cancer patients" vorgelegt von Dr.-Ing. Sebastian Günther
title_short	"Analyses of the urinary miRNA-expression of triple-negative breast cancer patients"
title_sort	analyses of the urinary mirna expression of triple negative breast cancer patients
topic_facet	Hochschulschrift
url	https://d-nb.info/1232033286/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033269126&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
work_keys_str_mv	AT gunthersebastian analysesoftheurinarymirnaexpressionoftriplenegativebreastcancerpatients

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